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KMID : 0644120120250020012
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Journal of Oriental Gynecology
2012 Volume.25 No. 2 p.12 ~ p.22
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Immune Enhancing Effect of Houttuyniae Herba on Mouse Macrophage
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Kim Jeong-Hyun
Kim Yoon-Sang
Lim Eun-Mee
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Abstract
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Objectives: The aim of this study is to investigate immune enhancing effect of Houttuyniae Herba water extract(HW) on RAW 264.7 cell of mouse macrophages.
Methods: Effects of HW on productions of nitric oxide(NO) and hydrogen peroxide(H₂O₂) in RAW 264.7 mouse macrophages were measured. Effect of HW on production of cytokines such as interleukin(IL)-1β, IL-6, and tumor necrosis factor(TNF)-α in RAW 264.7 cells was accessed by a multiplex bead array assay based on xMAP technology. All of results were represented P<0.05 compared to the normal.
Results: 1. After 24 hr incubation, HW increased significantly NO production in RAW 264.7 cells at the concentrations of 25, 50, 100 and 200㎍ /mL. 2. After 24 hr incubation, HW increased significantly hydrogen peroxide production in RAW 264.7 cells at the concentrations of 25, 50, 100 and 200 ㎍/mL. 3. After 24 hr incubation, HW increased significantly IL- 1β production in RAW 264.7 cells at the concentrations of 100 and 200 ㎍/mL. 4. After 24 hr incubation, HW increased significantly IL-6 production in RAW 264.7 cells at the concentrations of 100 and 200 ㎍/mL. 5. After 24 hr incubation, HW increased significantly TNF-α production in RAW 264.7 cells at the concentrations of 50, 100, and 200 ㎍/mL.
Conclusions: These results suggest that HW has immune enhancing activity related with its increasement of NO, hydrogen peroxide, IL-1β, IL-6, and TNF-α in macrophages.
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KEYWORD
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Houttuyniae Herba, macrophage, cytokine, hydrogen peroxide, NO
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